In recent years, there have been many reports of the treatment of osteoarthritis by biotherapy such as PRP and cultured stem cells. In this study, Conditioned medium of mesenchymal stem cell (CM MSC) was used for the treatment of osteoarthritis. Conditioned medium of Infant dental pulp stem cell (SHED-CM) and CM from bone marrow-derived stem cell (BMSC-CM) were administered intraarticularly to 9 patients with knee OA) and 9 patients with hip OA). VAS, KOOS, and JHEQ were evaluated and compared with before treatment. In hip OA, the patient responded to CM early and had a pain-relieving effect that lasted up to 6 months. Knee OA was effective in improving pain, but the effect was uneven. In the future, it is necessary to study the amount and type of cytokines and exosomes in CM-MSC, as well as the optimal dosage.

Key words Conditioned Medium，MSCs，Osteoarthritis, Biotherapy